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. 2020 Aug 13;10:13684. doi: 10.1038/s41598-020-70291-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of the labelling protocol on UCMSC differentiation is presented. (A) Adipogenic differentiation is detected by Oil red O staining; all normal. (B) Osteogenic differentiation is detected by Alizarin red S staining; all normal. (C) Chondrogenic differentiation is detected by Alcian blue staining; a small impairment is observed at 100 μg/mL of SPIO. Oil red O and Alizarin red S: scale bar, 100 µm; Alcian blue: scale bars, 200 µm. UCMSCs umbilical cord-derived mesenchymal stem cells, SPIO superparamagnetic iron oxide, USPIO ultrasmall superparamagnetic iron oxide.