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. 2020 Aug 13;11:4043. doi: 10.1038/s41467-020-17725-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Schematic illustrating the production of Cas9 double-ssODN conjugates. bHiBiT sequence knockin at the GAPDH locus was detected in U2OS cells (n = 4 biologically independent experiments). c, d Two-base exchange at the RBM20 locus was detected in HEK-293FT cells. Unlabeled wild-type Cas9 (wt) and Cas9-adaptor conjugates labeled at the indicated residues were used (n = 3 biologically independent experiments). P-values were calculated by paired two-tailed t-test. Source data are provided as a Source Data file.