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. 2020 Aug 13;11:4043. doi: 10.1038/s41467-020-17725-0

Fig. 5. Efficient engineering of INS-1E cells for secretion of exogenous peptides and proteins.

Fig. 5

a Schematic of genome editing in the Ins1 locus of INS-1E cells. PC, prohormone convertase. HDR, homology-directed repair. b Engineered cells can secrete exogenous gene products together with insulin. c INS-1E cells were engineered to secrete the 11-residue HiBiT peptide. Multiple gene insertion sites and DNA break sites were investigated (n = 2 biologically independent experiments). A.U. arbitrary unit. d INS-1E cells were engineered to secrete interleukin 10 (IL-10), (n = 2 biologically independent experiments). LPS lipopolysaccharide. RNP ribonucleoprotein. e, f Display of ssODN on Cas9 enhanced the secretion of e HiBiT peptide and f IL-10 (n = 3 biologically independent experiments). Source data are provided as a Source Data file.