FIGURE 2.

ILCs and ILC2s increased in the ipsilateral hemispheres in the neonatal mouse brain in a time-dependent fashion after HI injury. Single-cell suspensions generated from brain homogenates were analyzed using flow cytometry in mouse pups at different time points after HI, with naïve C57Bl6/J mouse littermates as controls (n = 6–8/group). (A) Representative flow cytometry plots showing the gating strategy for ILCs and ILC2s. Both are represented as percentages of the CD45⁺ population. ILCs (B) and ILC2s (C) in the ipsilateral and contralateral hemisphere at 24 h, 3 days, and 7 days after HI. A mixed model ANOVA with Games-Howell post-hoc were used. *#: p < 0.05, **##: p < 0.01, ***###: p < 0.001. in panels (B,C). Abbreviations: N: naïve, HI: hypoxia-ischemia, IP: ipsilateral hemisphere, CO: contralateral hemisphere.