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. 2020 Aug 13;10:13702. doi: 10.1038/s41598-020-70625-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Adipocytes are key cells expressing chemerin following stimulation with IL-1β + OSM. eWAT or SCA-1 + APs were isolated. The cells were differentiated to obtain a mature adipocyte cell culture. Then, the cells were treated with IL-1β (10 ng/mL), OSM (50 ng/mL), or a combination for up to five days. The levels of chemerin mRNA (A, C) were determined using RT-QPCR. The relative expression of stimulated cells over the control is shown. Levels of secreted chemerin were determined in parallel in conditioned media by ELISA (B, D). Data are presented as the mean ± SEM of at least three independent experiments. Statistical significance between the control and the treated cells is indicated by an asterisk; *p < 0.05 by analysis of covariance followed by a Bonferroni post-hoc test.