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. 2020 Aug 13;10:13702. doi: 10.1038/s41598-020-70625-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The proximal part of the chemerin promoter is a key regulator of transcription. The murine RARRES2 promoter regions (from − 735 to + 258 bp) were cloned into the pNL1.1[Nluc]-Basic vector (A). 3T3-L1 adipocyte precursors were used for transient transfections since these cells respond to IL-1β + OSM stimulation similarly to the primary adipocytes (B). 3T3-L1 cells were transfected with Chemerin_Full vector (C) or different chemerin promoter constructs (D, E) and eventually stimulated with cytokines. The results are expressed as the fold-change in relative luciferase units relative to the empty vector. Statistical significance is indicated by an asterisk; *p < 0.05 by ANOVA followed by a Bonferroni post-hoc test. The Mann–Whitney test was used to analyze statistical differences between methylated/mock methylated vectors. Data are presented as the mean ± SD of at least three independent experiments.