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. 2020 Aug 13;11(8):616. doi: 10.1038/s41419-020-02867-2

Fig. 3. The metabolic effects of venetoclax are independent of BCL-2 family proteins.

Fig. 3

a CT26 cells were treated for 24 h with ABT-737 (1 μM) and OCR was measured. Data represent the mean of five technical replicates from one representative experiment (out of two). b CT26 cells were treated for 24 h with S55746 (1 μM) and OCR was measured. Data represent the mean of five technical replicates from one representative experiment (out of two). c BCL-2 protein expression in CT26 CRISPR-EMPTY and CT26 CRISPR-BCL2 cells was determined by western blot, α-tubulin was probed as a loading control. d OCR of CT26 CRISPR-EMPTY and CT26 CRISPR-BCL2 cells. Data represent the mean of five independent experiments ± SEM. e Metabolite levels of CT26 CRISPR-EMPTY and CT26 CRISPR-BCL2 cells. Data represent the mean of three technical replicates. CT26 CRISPR-EMPTY and CT26 CRISPR-BCL2 cells were treated with venetoclax (1 μM) for 24 h; OCR was assessed (f), metabolites from the media were measured (g), and intracellular metabolites were extracted and analysed by LC–MS (h). Data represent the mean of three independent experiments ± SEM (f, g) or one independent experiment with three technical replicates (h). i Basal OCR of Bcl-2 allKO HCT116 cells after treatment with venetoclax (1 μM) for 24 h. Data represent the mean of four independent experiments ± SEM. Samples were compared using two-tailed, unpaired Student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001.