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. 2020 Aug 13;11:4072. doi: 10.1038/s41467-020-17889-9

Fig. 3. Genome-wide off-target sites of dLbCpf1-BE revealed by Digenome-seq and targeted deep sequencing.

Fig. 3

a, b Venn diagrams showing the number of in vitro cleavage sites in the human genome produced by dLbCpf1-BE or LbCpf1 nuclease targeting the DYRK1A (a) and DNMT1 (b) sites that were identified by Digenome-seq. c Sequence logos were generated for DNA sequences captured by dLbCpf1-BE- and LbCpf1-mediated Digenome-seq using WebLogo. d, e Base editing frequencies of dLbCpf1-BE at in vitro cleavage sites, identified by dLbCpf1-BE-mediated Digenome-seq, were measured using targeted deep sequencing of genomic DNA from HEK293T cells. Mismatched bases and PAM sequences are shown in red and blue, respectively. An RNA bulge is represented as a dash and a DNA bulge is shown in green. Data are shown as mean ± s.e.m. from three biologically independent samples.