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. 2020 Aug 13;9(8):72. doi: 10.1038/s41389-020-00257-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic diagram of PD-L1-CAR and CD19-CAR constructs. scFv for PD-L1 is derived from atezolizumab (Roche). b Surface expression of PD-L1-CAR on transduced T cells as measured by flow cytometry using Biotin-Protein L and APC-streptavidin on day 5 post transduction. c Viability of PD-L1-CAR T cells on day 7 and 14 post-transduction. d Expansion of PD-L1-CAR and CD19-CAR T cells in vitro for 14 days. e Percentage of CAR T cells that were positive for CD3, CD4, CD8, PD-1, PD-L1, and TIM3 on day 7 and 14. f Percentage of CAR T cells that were positive for memory cell markers on day 7 and 14. Data represented technical triplicates using T cells from one donor and were displayed as mean ± SEM. *p ≤ 0.05, ns not significant.