Fig. 2. PD-L1-CAR T cells kill NSCLC cells in a PD-L1-dependent manner.

a Flow cytometry histogram of the surface antigen expression of PD-L1 in human NSCLC cell lines and BEAS-2B. b Cytotoxic activity of PD-L1-CAR T cells after 4 and 20 h of co-culture with human NSCLC cell lines and BEAS-2B. PD-L1-CAR T cells and CD19-CAR T cells were used as effector cells at various ratios of effector (E): target (T). c Secretion of cytokines analyzed by ELISA in supernatants obtained after a 20-h co-culture of effector and target cells at a 2:1 E:T ratio. Data represented technical triplicates using T cells from one donor and were shown as mean ± SEM. ****p ≤ 0.0001.