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. 2020 Aug 13;10:13740. doi: 10.1038/s41598-020-70793-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Efficiency of lentiviral transduction and cell sorting. MACS enriched CD105⁺/Sca1⁺ BM-cells were transduced with an MOI of 50 using the dLck-lentivirus. (A) Flow cytometric analysis was performed 7 days later to determine expression efficiency. Oligopotent progenitor cells (OPP), short term HSC (ST-HSC), and long term HSCs (LT-HSC) show ubiquitous mCherry expression with a higher mCherry expression rate in ST-HSC. (B) mCherry expression is analyzed before (left) and after (right) FACS based cell sorting for transgene CD105⁺/Sca1⁺ BM-cells. (C) eGFP was not detectable on transduced mCherry⁺ CD105⁺/Sca1⁺ BM-cells compared to non-transduced cells.