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. 2020 Aug 13;10:13740. doi: 10.1038/s41598-020-70793-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Specificity of lentiviral constructs in peripheral blood. Eight to ten weeks post HSC-transplantation leukocyte subsets in peripheral blood were evaluated by flow cytometric analysis for CD3δ-lentivirus transduced HSCs (n = 5, A,B) or for dLck-lentivirus transduced HSCs (n = 9, C,D). Representative dot plots depicting eGFP and mCherry expression are shown for CD3⁺ T-cells (A,C, left), CD3⁻ non-T-cell PBMCs (A,C, middle) and CD3⁻ granulocytes (A,C, right). In (B,D) quantification of mCherry⁺ and GFP⁺ cells. Error bars indicating SD. *p < 0.05.