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. 2020 Aug 13;13:144. doi: 10.1186/s13068-020-01781-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Cell growth of recombinant and wild-type strains of Z. mobilis containing the control plasmid pEZ15A and plasmid constructs of pET-Tc3 and pET-Tc5 (M) at pH 3.6, 4.0, and 6.0 without tetracycline (a, c) and with 0.8 μg/mL tetracycline induction (b, d). pEZ-Tc3, plasmid construct expressing operon ZMO0956–ZMO0958 encoding cytochrome bc1 complex; pEZ-Tc5 (M), plasmid construct expressing operon ZMO1428–ZMO1432 encoding RND efflux system with a mutation in ZMO1432. At least two independent experiments were performed with similar results. Values are the mean of one representative experiment with three technical replicates. Error bars represent standard deviations