Table 2.
Single-nucleotide polymorphisms (SNPs) in mutant strains 3.5M and 3.6M compared to wild-type ZM4
| Locus | SNP | AA change | 3.5M | 3.6M | Gene | Product |
|---|---|---|---|---|---|---|
| 424761 | C/T | A67T | 99.76 | 98.86 | ZMO0421 (hisC2) | Histidinol-phosphate aminotransferase |
| 711194 | G/A | G539D | 99.24 | 99.73 | ZMO0712 (ppk) | Polyphosphate kinase |
| 1449594 | G/A | P480L | 100 | 99.72 | ZMO1432 | Efflux pump membrane component |
| 1779278 | C/A | T7K | 100 | 99.71 | ZMO1733 (oxyR) | Transcriptional regulator OxyR |
| 1306151 | C/T | W485* | 99.5 | – | ZMO1291 | Peptidase S10 serine carboxypeptidase |
| 1701191 | G/A | L77F | 99.08 | – | ZMO1651 (ptsP) | Signal transduction protein |
| 173653 | T/C | 100 | – | Intergenic region | Between ZMO0183 and ZMO0184 | |
| 1451222 | A/G | – | 100 | Intergenic region | Between ZMO1432 and ZMO1433 |
The numbers in the columns of 3.5M and 3.6M represent the frequency (%) of the SNP identified in all genome resequencing reads, and “–” indicates the absence of SNP. AA means amino acid. * indicates stop codon