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. 2020 Aug 13;10:13686. doi: 10.1038/s41598-020-70721-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of the death domain states by NMR. (A) Generalized chemical shift changes of cross-peaks in ¹H,¹⁵N-HSQC spectra between the D352 and isoD352 (on top) and between N352 and D352 (at bottom) states of hP75DD. (B) The structure of the death domain (PDB:2N97) is colored with respect to the chemical shift changes upon deamidation. Red are the residues with GCS changes greater than 0.1 ppm. (C) A set of three fragments of ¹H,¹⁵N-HSQC spectra of hP75DD in 50 mM phosphate buffer acquired at immediately, on the 6th day and 19th day after the sample preparation. Positions of Asn352 sidechain NH₂-group are shown, as well as of three well-resolved signals, corresponding to the different states of Thr377. The figure was prepared using the program Inkscape 0.92 (https://inkscape.org/).