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. 2020 Aug 13;10:13686. doi: 10.1038/s41598-020-70721-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of dimerization on the structure and behavior of the intracellular domain of rP75-ΔECD-3CX. (A) ¹H,¹⁵N-HSQC spectra of rP75-ΔECD-3CX monomer (shown in blue) and disulfide-crosslinked dimer (shown in red). The full spectrum and central region are shown at left and right, respectively. (B) NMR-derived rotational diffusion correlation time of N–H bonds is plotted versus the residue number of rP75-ΔECD-3CX monomer (blue bars) and dimer (red bars). The figure was prepared using the program Inkscape 0.92 (https://inkscape.org/).