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The cultivation conditions of E. coli for expression of rP75-ΔECD-3CX, rP75-ΔECD-3CX-249C, rP75DD and hP75DD proteins.
| rP75-ΔECD-3CX | rP75-ΔECD-3CX-249C | rP75DD | hP75DD | |
|---|---|---|---|---|
| IPTG, mM a | Without IPTG | 0.1 | 0.5 | 0.5 |
| Time, h b | 24–30 | 24–30 | 30 | 4 |
| Temp., °C c | 37 | 13 | 13 | 37 |
aFinal concentration of inductor, IPTG.
bThe time of cells growth after induction.
cThe temperature of cells cultivation after induction.
