Table 1.

Rate and dissociation constants from BLI experiments.

Variant	State measured	kon (μM−1 s−1)	koff (s−1)	Kd (μM)
Monobody HA4	–	0.0631	0.0145	0.23
OptoMB	Light conformation	<0.001	0.21 ± 0.09	>100
aOptoMBC450V	Dark conformation	0.071 ± 0.033	0.01 ± 0.004	0.19 ± 0.11
aOptoMBV416L	Light conformation	0.004 ± 0.001	0.23 ± 0.02	63 ± 23

^aAverage of three individual measurements ± SD.