Table 2.

Purification parameters of LCAC batch experiments.

Purified protein	OptoMB version	Resin type	Puritya (%)	Resin capacitya (mg/mL)	Yielda (% of recovery from the resin)
YFP-SH2	OptoMB	Talonb	98.25 (±2.26)	5.85 (±0.67)	17.8 (±2.18)
YFP-SH2	cOptoMBTriple	Talonb	95.32 (±0.46)	4.5 (±0.33)	29.79 (±3.28)
SH2-Pdc1p	cOptoMBTriple	Talonb	95.53 (±1.05)	5.24 (±0.52)	39.01 (±2.61)
YFP-SH2	cOptoMBTriple	CNBrd	99.78 (±0.22)	2.55 (±0.22)	40.29 (±3.02)
SH2-Pdc1p	cOptoMBTriple	CNBrd	96.69 (±1.24)	1.55 (±0.18)	42.28 (±4.34)

Values expressed as mean ± SD for n = 3 independent measurements. Source data are provided as a Source Data file.

^aSee “Methods” for calculations.

^bOptoMB was His-tag bound.

^cSUMO tagged.

^dOptoMB was covalently conjugated.