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. Author manuscript; available in PMC: 2020 Nov 7.
Published in final edited form as: J Phys Chem Lett. 2019 Oct 21;10(21):6807–6812. doi: 10.1021/acs.jpclett.9b02250

Figure 3.

Figure 3.

Schematic representation (a) of the optical setup for high-throughput SMS and representative frames capturing the locations and emission spectra of individual molecules concurrently. Single-molecule emission spectra recorded over time (b), averaged emission spectrum before photobleaching (c), intensity and spectral-centroid evolution (d) of a single BO molecule over a time course of 18 s and scatterplot (e) of the emission intensity against the spectral centroid of the single molecule measured in each consecutive frame before photobleaching.