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. 2020 Jul 1;40(8):355–369. doi: 10.1002/cac2.12069

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© 2020 The Authors. Cancer Communications published by John Wiley & Sons Australia, Ltd. on behalf of Sun Yat‐sen University Cancer Center

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Association of TESC with HCC cell growth. a. Western blotting analysis of TESC expression in different liver cancer cell lines. b. Western blotting analysis of cell lysates of HEK293T transfected with Flag‐tagged TESC and 3 pairs of TESC shRNA (#1‐#3) or the control shRNA (Ctrl) after 36 hours with indicated antibody. c, d. RT‐qPCR (c) and Western blotting (d) analyses of BEL‐7404 and SMMC‐7721 cells infected with lentiviruses containing TESC shRNA (shTESC‐3) or the control shRNA (shCtrl). e. Celigo cell counting of BEL‐7404 and SMMC‐7721 cells infected with lentiviruses containing shTESC‐3 or shCtrl. f. WST‐1 assay of BEL‐7404 and SMMC‐7721 cells infected with lentiviruses containing shTESC‐3 or shCtrl. *: P < 0.050; **: P < 0.010. TESC: Tescalcin; HCC: hepatocellular carcinoma; shRNA: short‐hairpin RNA; RT‐qPCR: real‐time quantitative polymerase chain reaction