REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
NEK7 Antibody	Abcam	ab133514
V5 Antibody	Life technologie Europe BV	R960-25
Anti-HA Antibody	Lucerna Chemie AG	HA-11, 901513
secondary anti-rabbit IgG HRP-conjugated antibody	Cell Signaling	#7074S; RRID:AB_2099233
Alexa Fluor® 488 anti-Vimentin	Biolegend	#677809; RRID:AB_2650955
Alexa Fluor® 647 anti-Tubulin Beta 3	Biolegend	#657406; RRID:AB_2563610
DAPI	Sigma Aldrich	N/A
Chemicals, Peptides, and Recombinant Proteins
Strep-Tactin Sepharose	IBA	2-1201-010
X-Treme Gene 9 DNA Transfection Reagent	Roche	06365787001
Hygromycin B	Invitrogen	10687010
BlasticidinS Hcl, 50 mg	Life Technologies	R21001
Biotin	Sigma	B4501-1G
Doxycycline Hyclate	Sigma	D9891-5G
Critical Commercial Assays
jetPRIME transfection Reagent	Chemie Brunschwig AG	114-15
Lipofectamine RNAiMAX	Invitrogen	13778075
Adriamycin	Sigma	D1515
Deposited Data
Processed Protein-Protein Interaction data	This paper	Table S2
Raw Mass Spectrometry Data and Peptide Identifications	This paper	Peptide Atlas: PASS01469 https://db.systemsbiology.net/sbeams/cgi/PeptideAtlas/PASS_View?identifier=PASS01469.
Experimental Models: Cell Lines
Human: Flp-Ln T-Rex 293 Cell Line	Thermo Fisher Scientific (Invitrogen)	R78007
Human: A549 Cell Line	ATCC	ATCC® CCL-185
Human: T-REx-HeLa CCL2 Flp-In cells	Thermo Fisher Scientific (Invitrogen)	R71407
Human: LN-229 (glioblastoma cell line)	ATCC	ATCC® CRL-2611
Human: SKOV3 (ovarian cancer cell line)	ATCC	ATCC® HTB-77
Oligonucleotides
Silencer select GSG2 siRNA	ThermoFisher	s38320
Silencer select PIM3 siRNA	ThermoFisher	s53946
Silencer select PIM3 siRNA	ThermoFisher	s53947
Silencer select PKMYT1 siRNA	ThermoFisher	s194986
Silencer select PKMYT1 siRNA	ThermoFisher	s194985
Silencer select CSNK1G1 siRNA	ThermoFisher	s28823
Silencer select CSNK1G1 siRNA	ThermoFisher	s28822
Silencer select WNK3 siRNA	ThermoFisher	s35278
Silencer select WNK3 siRNA	ThermoFisher	s35279
Silencer select POMK siRNA	ThermoFisher	s534531
Silencer select POMK siRNA	ThermoFisher	s534532
Silencer select NEK7 siRNA	ThermoFisher	s44315
Silencer select NEK7 siRNA	ThermoFisher	s44316
Silencer select NEK6 siRNA	ThermoFisher	s21184
Silencer select NEK6 siRNA	ThermoFisher	s21185
Silencer select RIOK2 siRNA	ThermoFisher	s622
Silencer select SCYL3 siRNA	ThermoFisher	s32780
Silencer select SCYL3 siRNA	ThermoFisher	s32778
Silencer select NEK9 siRNA	ThermoFisher	s40772
Silencer select NEK9 siRNA	ThermoFisher	s40771
siRNA Positive control: Silencer Cy3-labeled GAPDH siRNA	ThermoFisher	Catalog #: AM4649
siRNA Negative control: Silencer Select Negative Control No. 2 siRNA	ThermoFisher	Catalog #: 4390846
Recombinant DNA
hORFeome V5.1	Horizon Discovery/ Dharmacon	Open Biosystem
hORFeome V8.1	Horizon Discovery/ Dharmacon	The CCSB ORFeome Collection
pTOSH-GW-FRT-HA-Strep	Glatter et al., 2009	N/A
pOG44 Flp recombinase expression vector	Invitrogen	V600520
Kinome Constructs	Varjosalo et al., 2008	N/A
Software and Algorithms
Cytoscape 3.6.0	Kohl et al., 2011	https://www.cytoscape.org/
X!Tandem	Craig & Beavis, 2004	https://www.thegpm.org/tandem/
Abacus	Fermin et al., 2011	http://abacustpp.sourceforge.net/
ProteinProphet	Deutsch et al., 2010	Trans Proteomic Pipeline (TPP, v.4.6.0)
R package GOSemSim	Yu et al., 2010	Bioconductor (v. 2.6.2)
R package RDAVIDWebService	Fresno and Fernández, 2013	Bioconductor
NetPhorest	Miller et al., 2008	http://netphorest.info
CellProfiler 2.2.0	McQuin et al., 2018	https://cellprofiler.org
MATLAB R2019a		https://www.mathworks.com/
CompPASS	Sowa et al., 2009	http://besra.hms.harvard.edu/ipmsmsdbs/cgi-bin/tutorial.cgi
Other
Calculation of co-purification p values	This paper	N/A
Opera Phenix automated spinning-disk confocal microscope at 20x magnification	Perkin Elmer	HH14000000
Upon acceptance, we will share all images generated for the Manuscript through Mendeley Data		N/A