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. 2020 Aug 6;182(3):625–640.e24. doi: 10.1016/j.cell.2020.06.026

Figure 4.

Figure 4

Brain CD4 T Cells Are Licensed For Brain Entry by Peripheral Activation

(A–D) Perfused brains from Nur77-GFP reporter mice were assessed for Nur77 reporter expression, n = 8. (A) Representative histograms and (B) average expression for conventional CD4 T cells in the blood and brain. (C) Representative histograms and (D) average expression for Tregs in the blood and brain.

(E and F) Blood, perfused brains, spleen, and perfused lung from 2D2 transgenic mice and (right panel) wild-type controls were assessed for the frequency and numbers of transgene-expressing (Vα3.2+Vβ11+) and transgene-non-expressing (Vα3.2−Vβ11−) (E) conventional and (F) Tregs (n = 5,5).

(G and H) Blood, perfused brains, spleen, and perfused lung were collected from OT-II and (right panel) wild-type mice, on the Rag-sufficient or -deficient background, were assessed for the frequency and numbers of transgene-expressing (Vα2+Vβ5+) and transgene-non-expressing (Vα2−Vβ5−) (G) conventional and (H) Tregs (n = 5,5). p values represent one-way ANOVA with Tukey’s multiple comparison for cross-organ data and Sidak’s multiple comparison test for 2-way ANOVA for within brain comparison.

See also Figure S4.