Figure 5.

Brain Conventional CD4 T Cells Are Expanded by Exposure to the Microbiome

(A and B) Perfused mouse brains were compared to blood by high-dimensional flow cytometry from gnotobiotic, SPF, and dirty co-housed mice (n = 6,10,12). CD4 T cells in perfused brain as (A) a percentage of CD45⁺ cells and (B) absolute numbers of CD4 T cells in the brain.

(C and D) t-SNE of conventional (C) T cells and (D) Tregs built on CD62L, CD44, CD103, CD69, CD25, PD-1, Nrp1, ICOS, KLRG1, ST2, Ki67, Helios, T-bet, and CTLA4, with quantified FlowSOM clusters. p values represent cross-entropy comparison to SPF mice.

See also Figure S5.