Figure S4.

Phenotypic Profile of Brain-Resident TCR Transgenic CD4 T Cells, Related to Figure 4

CD4 T cells from perfused brains of TCR transgenic mice and wild-type controls (n = 5) were assessed by high parameter flow cytometry for CD62L, CD44, CD103, CD69, CD25, PD-1, Nrp1, ICOS, KLRG1, ST2, Ki67, Helios, T-bet, CTLA4. (A) tSNE of CD4⁺Foxp3^- conventional 2D2 T cells, gated on CD4⁺Foxp3^-CD3⁺CD8^-CD45⁺ cells and subdivided into transgene-expressing (Vα3.2⁺Vβ11⁺) and transgene-non-expressing (Vα3.2^-Vβ11^-) cells. The tSNE was built on CD62L, CD44, CD103, CD69, CD25, PD-1, Nrp1, ICOS, KLRG1, ST2, Ki67, Helios, T-bet, CTLA4. FlowSOM clusters are illustrated in color and (B) quantified. (C) Heatmap of expression changes between transgene-expressing (Vα3.2⁺Vβ11⁺) and transgene-non-expressing (Vα3.2^-Vβ11^-) conventional 2D2 CD4⁺ T cells. (D) tSNE of CD4⁺Foxp3⁺ 2D2 regulatory T cells, gated on CD4⁺Foxp3⁺CD3⁺CD8^-CD45⁺ cells and subdivided into transgene-expressing (Vα3.2⁺Vβ11⁺) and transgene-non-expressing (Vα3.2^-Vβ11^-) cells. The tSNE was built on CD62L, CD44, CD103, CD69, CD25, PD-1, Nrp1, ICOS, KLRG1, ST2, Ki67, Helios, T-bet, CTLA4. FlowSOM clusters are illustrated in color and (E) quantified. (F) Heatmap of expression changes between transgene-expressing (Vα2⁺Vβ5⁺) and transgene-non-expressing (Vα2^-Vβ5^-) regulatory T cells. (G) tSNE of CD4⁺Foxp3^- conventional OT-II T cells, gated on CD4⁺Foxp3^-CD3⁺CD8^-CD45⁺ cells and subdivided into transgene-expressing (Vα2⁺Vβ5⁺) and transgene-non-expressing (Vα2^-Vβ5^-) cells. The tSNE was built on CD62L, CD44, CD103, CD69, CD25, PD-1, Nrp1, ICOS, KLRG1, ST2, Ki67, Helios, T-bet, CTLA4. FlowSOM clusters are illustrated in color and (H) quantified. (I) Heatmap of expression changes between transgene-expressing (Vα2⁺Vβ5⁺) and transgene-non-expressing (Vα2^-Vβ5^-) conventional OT-II CD4⁺ T cells.