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. 2020 Aug 7;11:1875. doi: 10.3389/fmicb.2020.01875

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Copyright © 2020 Ng, Ong, Surendran, Sinha, Lai, Chen, Liang, Tay, Cui, Loo, Ho, Han and Moreira.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Exogenous H₂S sensitizes A. baumannii to killing by antibiotics. Time-kill experiments were conducted with two different isolates of A. baumannii, ATCC #2093 (A–D) and clinical isolate #8879 (E–H). Briefly, 10⁶ bacteria were treated with either a sub-lethal concentration of antibiotic alone or combined with the H₂S-releasing molecule NaHS (sodium hydrosulfide) at given concentrations. Survival was determined by colony enumeration on agar plates at indicated time points. Gm, Gentamicin; Col, Colistin; Rif, Rifampicin; Clr, Clarithromycin. Experiments were carried in two independent biological replicates. Representative results are shown.