Figure 3.

Targets of miR-206/−1a are specifically upregulated in differentiating triple KO clones and are not affected by differentially expressed miRNAs.

A) Top: Cumulative distribution function (CDF) plot showing no change of the miR-206/−1 targets in proliferating triple KO clones (KO) versus control cells (Ctrl). The curves for the microRNA targets and non-targets are virtually superimposed. Bottom: Histogram of fraction of the miR-206/−1 targets, among all bins arranged from the most repressed to most induced in tKO vs Ctrl cells in growth medium. The genes are ranked from the most downregulated (blue) to the most upregulated (red). The horizontal line depicts the uniform distribution expected under the null hypothesis. Targets are based on match to 7mer sequence.

B) Top: Cumulative distribution function (CDF) plot showing upregulation of miR-206/−1 targets in differentiating triple KO clones (KO) versus differentiating control cells (Ctrl). Bottom: Histogram similar to that in Figure 3A, but in differentiating medium.

C) MA plots to identify differentially expressed miRNAs between control cells and triple KO clones in proliferation (top) and differentiation conditions (bottom). The microRNA abundance was measured by small RNA-seq. Upregulated microRNAs are presented in blue and downregulated in red (padj<0.1).