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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: FASEB J. 2020 Apr 11;34(6):7687–7702. doi: 10.1096/fj.201902855RR

Figure 4.

Figure 4.

Knockout of miR-206, miR-1a-1 and miR-1a-2 leads to partial embryonic lethality and diminishes adult mice physical potential

A) Scheme of animal crosses leading to generation of triple KO mice.

B) Genotypes of offspring generated from miR-206 KO miR-1a-1 HET miR-1a-2 intercrosses. In total 127 animals were genotyped. Percentage of expected and observed genotypes are given for weaning-age (3-week-old) pups. (**), (*) indicates p-value =< 0.01, =<0.05.

C) qRT-PCR analysis of microRNAs in TA skeletal; muscles from control wild-type (WT, n=3) and triple KO (tKO, n=2) animals. Levels of miRNAs were normalized to U6 snRNA. Levels are shown relative to WT. Values presented as mean +/− SD. Statistical significance was calculated using t-student test. (***) indicates p-value =< 0.001.

D) Animal weight used for physical performance tests. Statistical significance was calculated using t-student test. (***) indicates p-value =< 0.001. (*) indicates p-value =< 0.05. N = 13 for all genotypes, except for miR-206&1–1&1–2 tKO (N = 2).

E) Forelimb grip strength measured using grid normalized to respective body weights. Values presented as mean +/− SD. Statistical significance was calculated using t-student test. (***) indicates p-value =< 0.001 in comparison to WT mice. (†††), (††), (†) indicates p-value =< 0.001, =<0.01, =< 0.05 respectively in comparison to tKO mice. N = 13 for all genotypes, except for miR-206&1–1&1–2 tKO (N = 2).

F) Top: Latency to fall measured using rotating rod. Values presented as mean +/− SD. N = 13 for all genotypes, except for miR-206&1–1&1–2 tKO (N = 2). Bottom: Statistical significance heatmap calculated using one-way ANOVA test.

G) Maximum speed of rotation tolerated by the animals measured using rotating rod at the end of experiment (3rd day). Values presented as mean +/− SD. Statistical significance was calculated using t-student test. (***), (**) indicates p-value =< 0.001, =<0.01 respectively in comparison to WT mice. (†††), (†) indicates p-value =< 0.001, =< 0.05 respectively in comparison to tKO mice. N = 13 for all genotypes, except for miR-206&1–1&1–2 tKO (N = 2).