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. 2020 Aug 7;11:1944. doi: 10.3389/fmicb.2020.01944

TABLE 2.

Antimicrobial concentrations included on FASTvet (breakpoint values according CLSI).

Antimicrobial FASTvet panel concentrations (μg/ml)
Phenotype S I R S (urine) R (urine)
Gram negative bacilli Ampicillin ≤0.25 0.5 >0.5 ≤8 >8
Amoxicillin-clavulanic acid ≤0.25/0.12 0.5/0.25 >0.5/0.25 ≤8/4 >8/4
Piperacillin-tazobactam* ≤16/4 64/4 >64/4
Cefpodoxime ≤2 4 >4
Imipenem* Enterobacterales ≤1 2 >2
Imipenem* Pseudomonas spp. ≤2 4 >4
Enrofloxacin Enterobacterales ≤0.12 0.25 >0.25
Enrofloxacin Pseudomonas spp. ≤0.5 2 >2
Gentamicin ≤2 4 >4
Amikacin ≤4 8 >8
Staphylococcus spp. Penicillin* ≤0.12 >0.12
Cefoxitin* ≤4 >4
Enrofloxacin ≤0.5 2 >2
Vancomycin* S. aureus ≤2 8 >8
Enterococcus spp. Vancomycin* S. coagulase neg ≤4 16 >16
Ampicillin* ≤8 >8
Vancomycin* ≤4 16 >16

*Human breakpoints. According to flow cytometry analysis when the cells show lesions with the lowest concentration tested, the strain is considered S (susceptible); if no lesion is shown with the lower concentration and but only with the second concentration, the strain is considered I (intermediate); if even with the highest concentration no effect is seen, the strain is considered R (resistant). The category of I is not always present.