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TPPU upregulated the expression of occludin, ZO-1 and claudin-5 in rat pMCAO model. (A) Western blotting images. (B–D) Densitometric analysis of protein bands (n = 6 for each group). GAPDH served as the loading control. #P < 0.01 vs. the sham group; *P < 0.05, **P < 0.01 vs. the pMCAO group. pMCAO, permanent middle cerebral artery occlusion; TPPU, 1-trifluoromethoxyphenyl-3-(1-propionylpiperidin-4-yl) urea; ZO-1, Zonula occludens-1; GAPDH, glyceraldehyde-3-phosphate.
