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. 2020 Aug 7;10:1551. doi: 10.3389/fonc.2020.01551

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Copyright © 2020 Charazac, Fayyad, Beal, Bourgoin-Voillard, Seve, Sauvaigo, Lamartine, Soularue, Moratille, Martin, Ravanat, Douki and Rachidi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NBCCS fibroblasts are specifically more sensitive to ionizing radiation and UVA-induced cytotoxicity. Long-term survival curves determined by clonogenic assays. Cells were plated at low density (20 cells/cm²) and the second day they were exposed to growing dose of (A) ionizing radiation (γ Rays), (B) UVA radiation, (C) UVB radiation or (D) bleomycin and cultured for 2 weeks. Cells were then fixed with ethanol, stained with crystal violet in order to count the number of colonies. Results are expressed as mean percentage of survival fraction ± SD of colonies of 3 independent experiments (n = 3) and triplicate measurements. μ GM01552 significantly (p < 0.05) different from the normal cells (GM00730) stressed at the same dose. $ GM02098 significantly (p < 0.05) different from the normal cells (GM00730) stressed at the same dose. $ GM03300 significantly (p < 0.05) different from the normal cells (GM00730) stressed at the same dose.