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. 2020 Aug 7;10:1551. doi: 10.3389/fonc.2020.01551

FIGURE 2.

FIGURE 2

Expression of BER and DNA damage signaling-associated genes in NBCCS fibroblasts at basal level. Gene expression was investigated in Gorlin vs. control fibroblasts at basal level by RT-qPCR experiments. After total RNA extraction of each cell culture, they were retro-transcribed in complementary DNA. 20 ng of cDNA were used to detect specific gene expression. Three independent experiences were realized (n = 3). The calibrator used is non-irradiated GM00730. The upper panel reports results for the BER genes while the lower one shows data for the DNA double strand breaks repair. Collapse of BER glycosylases gene expression as well as DNA Damage Response gene signaling is shown here. No statistical differences in the expression of the polymerase beta was found. * Significantly (p < 0.05) different from the normal cells (GM00730).