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. 2020 Aug 7;10:1551. doi: 10.3389/fonc.2020.01551

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Copyright © 2020 Charazac, Fayyad, Beal, Bourgoin-Voillard, Seve, Sauvaigo, Lamartine, Soularue, Moratille, Martin, Ravanat, Douki and Rachidi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of BER and DNA damage signaling-associated genes in NBCCS fibroblasts after irradiation. Gene expression was investigated by RT-qPCR experiments in NBCCS vs. control fibroblasts at 4 h after 6 Gy ionizing irradiation. After total RNA extraction of each cell line, they were retro-transcribed in complementary DNA. 20 ng of cDNA were used to detect specific gene expression. Three independent experiences were realized (n = 3). The calibrator use is non-irradiated GM00730. The upper panel reports results for the BER genes while the lower one shows data for the DNA double strand breaks repair. Collapse of BER glycosylases gene expression as well as DNA Damage Response gene signaling are demonstrated here. No statistical differences in the expression of the polymerase beta was found. Furthermore, gene expression of the normal cell line was decreased after a stress. *Significantly (p < 0.05) different from the irradiated normal cells (GM00730).