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. Author manuscript; available in PMC: 2020 Aug 14.
Published in final edited form as: Nat Protoc. 2020 Feb 12;15(3):750–772. doi: 10.1038/s41596-019-0247-2

Table 1 |.

DNA oligonucleotide sequences

Oligo name Sequences Purpose Associated steps
CellTag-V1 gBlock 5′-ACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAACCGGTNNNNNNNNGAATTCGATGACAGGCGCAGCTTCCGAGGGATTTGAGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCAAAACTAGAATGCAGTGAAAAAAATGCCTTATTTGTGAAATTTGTGA-3′ Generation of complex CellTag libraries 1–5
Forward CellTag sequencing primer 5′-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT CATGGACGAGCTGTACAAGTAA-3′ Assessment of CellTag library complexity 19–31
Reverse CellTag sequencing primer 5′-CAAGCAGAAGACGGCATACGAGATACAGTGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGTGCAGGGGAAAGAATAGTAGAC-3′ Assessment of CellTag library complexity 19–31