CellTag-V1 gBlock |
5′-ACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAACCGGTNNNNNNNNGAATTCGATGACAGGCGCAGCTTCCGAGGGATTTGAGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCAAAACTAGAATGCAGTGAAAAAAATGCCTTATTTGTGAAATTTGTGA-3′ |
Generation of complex CellTag libraries |
1–5 |
Forward CellTag sequencing primer |
5′-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT CATGGACGAGCTGTACAAGTAA-3′ |
Assessment of CellTag library complexity |
19–31 |
Reverse CellTag sequencing primer |
5′-CAAGCAGAAGACGGCATACGAGATACAGTGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGTGCAGGGGAAAGAATAGTAGAC-3′ |
Assessment of CellTag library complexity |
19–31 |