| Reagent | Amount |
|---|---|
| 5× PCR buffer | 4 μl |
| 10 mM dNTP mix | 0.4 μl |
| 5 ng CellTag-V1 gBlock | x μl |
| 100 ng pSMAL destination plasmid | x μl |
| Phusion polymerase | 0.2 μl |
| Water | To 20 μl |
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