| Temperature | Time | No. of cycles |
|---|---|---|
| 95 °C | 3 min | 1 |
| 95 °C | 20 s | 12 |
| 65 °C | 15 s | |
| 72 °C | 20 s | |
| 72 °C | 1 min | 1 |
| 4 °C | Forever |
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