Skip to main content
. 2020 Aug 7;11:1579. doi: 10.3389/fimmu.2020.01579

Figure 2.

Figure 2

ADAM10 does not play a role in alpha-toxin-mediated death of Th1 cells. Murine conventional CD4+ T cells were differentiated into Th1 or Th17 cells and analyzed for ADAM10 expression by flow cytometry. Representative histogram and the summarizing graph show expression level of ADAM10 relative to FMO control. Cells were gated on living cells (A). Murine naïve CD4+ T cells were differentiated into Th1 cells in presence of indicated concentrations of alpha-toxin and in presence or absence of the ADAM10 inhibitor GI254023X. On day 4 of the culture, survival of the cells was analyzed by flow cytometry. Summarizing graph shows frequencies of living cells under the different conditions (B). Data were pooled from 4–9 independent experiments with 2–3 technical replicates. For statistical analysis, Two-way ANOVA, followed by Bonferroni multiple comparisons was applied. gMFI - Geometric mean fluorescence intensity. *p < 0.05, **p < 0.01.