Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 14;183(1):158–168.e14. doi: 10.1016/j.cell.2020.08.017

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 The Author(s)

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

PMC Copyright notice

Figure S3 — Immune Activation Patterns in Acute COVID-19, Related to Figure 2

(A) Representative flow cytometry plots showing the expression of activation/cycling markers among memory CD8⁺ T cells in patients with acute severe COVID-19. Numbers indicate percentages in the drawn gates. (B) Top left: UMAP plots showing the clustering of memory CD8⁺ T cells by phenotype in relation to all memory CD8⁺ T cells (left). Bottom left: UMAP plots showing the expression of individual markers (n = 1 donor per group). Right: dot plot summarizing the frequencies of CD38⁺ HLA-DR⁻ memory CD8⁺ T cells by group. Each dot represents one donor. Data are shown as median ± IQR. 2020 BD: healthy blood donors from 2020 (n = 18). AM: patients with acute moderate COVID-19 (n = 11). AS: patients with acute severe COVID-19 (n = 17). ^∗∗∗p < 0.001. Kruskal-Wallis rank-sum test with Dunn’s post hoc test for multiple comparisons. (C) Representative flow cytometry plots showing the expression of activation/cycling markers among CMV-specific memory CD8⁺ T cells in a healthy control and a patient with acute severe COVID-19. Numbers indicate percentages in the drawn gates. (D) Representative flow cytometry plots showing the phenotype of Ki-67⁺ memory CD8⁺ T cells in a patient with acute severe COVID-19. Numbers indicate percentages in the drawn gates. (E) Left: representative flow cytometry plots showing SARS-CoV-2-specific tetramer⁺ CD8⁺ T cells in two donors from group MC. Right: dot plot summarizing the frequencies of SARS-CoV-2-specific tetramer⁺ CD8⁺ T cells in donors from groups MC (n = 10) and SC (n = 2).