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. Author manuscript; available in PMC: 2020 Aug 14.
Published in final edited form as: Cell Rep. 2020 Jul 21;32(3):107917. doi: 10.1016/j.celrep.2020.107917

Figure 2. Integrator Mediates Transcriptional Elongation.

Figure 2.

(A) Spearman correlation of RNAPII and INTS11 ChIP-seq occupancy centered on the TSS (CAGE peak ±200 bp) of 8,000 genes.

(B) Hypothetical schematic of control condition “A” whereby treatments producing conditions “B” and “C” generate identical traveling ratio (TR) values by distinct transcriptional mechanisms.

(C and D) TM of control shRNA cells (C) and INTS11 shRNA cells (D).

(E) Differential TM of INTS11 shRNA cells.

(F and G) ChIP-seq promoter profiles of INTS11 (F) and RNAPII (G) centered on the +1 nucleosome dyad at gene classes defined by the differential TM. Kolmogorov-Smirnov (KS) test p value < 0.001 for classes paired against class IV.

(H and I) PRO-seq examples of class IV genes SEL1L (H) and MED4 (I) in control shRNA cells and INTS11 shRNA cells without and with shRNA induction.