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. Author manuscript; available in PMC: 2020 Aug 14.
Published in final edited form as: Cell Rep. 2020 Jul 21;32(3):107917. doi: 10.1016/j.celrep.2020.107917

Figure 3. Integrator Relieves RNAPII Pausing Adjacent to the +1 Nucleosome Dyad.

Figure 3.

(A and B) PRO-seq promoter-proximal read density and gene body profile at class I and II genes in control shRNA cells (A) and INTS11 shRNA cells (B) without and with shRNA induction. KS test p value = 0.01 of class I promoter and gene body profiles (−Dox versus +Dox). KS test p value <0.001 of class II promoter and gene body profiles (−Dox versus +Dox).

(C and D) PRO-seq promoter-proximal read density and gene body profile at class III and IV genes in control shRNA cells (C) and INTS11 shRNA cells (D) without and with shRNA induction. KS test p value < 0.001 for class III and IV (−Dox versus +Dox) promoter and gene body profiles.

(E) RNAPII ChIP-seq promoter and gene body profiles at class I and II genes without and with INTS11 shRNA induction. KS test p value < 0.05 and 0.01 for class I and II (−Dox versus +Dox) promoter profiles, respectively, and KS test p value < 0.05 for gene body profiles.

(F) RNAPII ChIP-seq promoter and gene body profiles at class III and IV genes without and with INTS11 shRNA induction. KS test p value < 0.0001 and 0.05 for class III and IV (−Dox versus +Dox) promoter profiles, respectively, and KS test p value < 0.001 for gene body profiles.

(G and H) Mononucleosome profile at class I and II genes in control shRNA cells (G) and INTS11 shRNA cells (H) without and with shRNA induction.

(I and J) Mononucleosome profile at class III and IV genes in control shRNA cells (I) and INTS11 shRNA cells (J) without and with shRNA induction.