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. Author manuscript; available in PMC: 2020 Aug 14.
Published in final edited form as: Cell Rep. 2020 Jul 21;32(3):107917. doi: 10.1016/j.celrep.2020.107917

Figure 5. INTS11 Catalyzes the Termination of Small RNA Associated with Paused RNAPII.

Figure 5.

(A) smRNA whole gene profile of all expressed genes (n = 8000) in INTS11 shRNA cells.

(B and C) Boxplot of log2(+Dox/−Dox) smRNA counts per million (CPM) at (B) Class IV and (C) Class III genes, t test; ***p value < 0.001.

(D) Boxplot of smRNA read length at all genes in INTS11 shRNA cells expressing WT-INTS11 and INTS11 shRNA cells expressing E203Q-INTS11. t test; ***p value < 0.001.

(E and F) smRNA mean size distribution centered on the TSSs of class IV (E) and class III (F) genes in control shRNA cells and INTS11 shRNA cells without and with shRNA induction.

(G and H) smRNA mean size distribution centered on the TSSs of class IV (G) and class III (H) genes in INTS11 shRNA cells expressing WT-INTS11 and INTS11 shRNA cells expressing E203Q-INTS11 without and with shRNA induction.

(I–L) smRNA read length distribution at all genes in control shRNA cells (I), INTS11 shRNA cells (J), INTS11 shRNA cells expressing WT-INTS11 (K), and INTS11 shRNA cells expressing E203Q-INTS11 (L) without and with shRNA induction.

(M and N) smRNA examples of class IV genes SEL1L (M) and MED4 (N) in control shRNA cells, INTS11 shRNA cells, INTS11 shRNA cells expressing WT-INTS11, and INTS11 shRNA cells expressing E203Q-INTS11 without and with shRNA induction.