Figure 2.

Treatment with NVP attenuates PDCD10 knockdown-induced migration of GBM cells. shPDCD10-GBM cells were treated with NVP (10 nM) for different time periods as indicated in individual experiments. EV served as control. (A) Scratch assay: cells were treated with 10 nM of NVP for 24 h followed by the scratching. The migration was recorded 24 h after scratching. (B) Spheroid migration assay: the spheroids formed by GBM cells were suspended in normal medium and seeded on 96-well-plates precoated with matrigel. After treatment with NVP (25 nM) for 48 h, the spheroids were collected and their diameter was measured. Scale bar: 100 μm. (C) Statistical analysis of scratch assay and spheroid migration assay. **P < 0.01, compared with EV; ^#P < 0.05, ^##P < 0.01, compared with shPDCD10.