Figure 3.

Treatment with NVP inhibits PDCD10 knockdown-induced adhesion and invasion of GBM cells. The adherent U87- or T98g cells were detected by crystal-violet staining after treatment of NVP (25 nM, 90 min) following by the absorbance measurement at 550 nm (A,C). The invasion of U87- or T98g cells were evaluated by the trans-well assay after treatment of NVP (25 nM for 48 h), the invaded cells were stained by crystal-violet and the absorbance was measuring at 550 nm (B,C). All data presented were representative of at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 compared with EV; ^#P < 0.05, ^##P < 0.01, compared with shPDCD10. Scale bar: 100 μm.