Fig. 5.

Glucose inhibition of Cs4130 in glucose and xylose mixtures. a High-cell density fermentation of Cs4130 and Gxf1 in xylose/glucose co-fermentation. Xylose and glucose consumption are represented by full and dotted lines, respectively. Fermentations were conducted on complete medium with 10 g/L of xylose and 10 g/L of glucose at 30 °C, 200 rpm. Control represents the EBY_Xyl1 harboring empty vector pRS426. Error bars represent standard deviation from average measures from triplicates. b Drop-assay analysis of EBY_Xyl1_hxk⁰ strain expressing the indicated transporters. Cells were grown on complete medium without uracil supplemented with 3% ethanol for 56 h before a serial dilution. The initial OD₆₀₀ was established on 1. Plates were incubated at 30 °C for 10 days