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Alignment of amino acid sequences of Cs4130, Gxf1, STP10, XylE and GlcPSE (a) and superposition of XylE (pale pink) and Cs4130 (pale green) intermembrane residues related to xylose (green) transport (b). The residues mutated in XylE and their counterparts in Cs4130, Gxf1, STP10, and GlcPSE are highlighted in green. The position of α-helix was signed based in XylE. α, α-helix; iα, intracellular α-helix
