Fig. 2.

Impact of cytokine activation on MSC lineage genes in UC and BM-MSCs. a A small cohort of Roson-Burgo MSC lineage genes is undetected before (5/445) or after (4/445) cytokine stimulation. Of these, DACT1, ADAMTS5, and LAMA4 are silenced in a cytokine-specific manner. Fifteen genes are significantly downregulated after conditioning by TNF-α or IFN-γ but remain detectable. b Four Rohart MSC test genes are consistently expressed in naïve and activated UC and BM-MSCs. In addition, GDF5 is downregulated but still measurable in all primed MSC populations. C1orf115 was also consistently expressed, except in one IFN-γ primed UC-MSC population. CES1P1 and HSPB3 were never detected, while OMD, ABI3BP, and SAA2 are only detected in some licensed populations, predominantly BM-MSCs. *p < 0.05 and > 2-fold change