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. 2020 Aug 14;13:418. doi: 10.1186/s13071-020-04281-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — The BI phylogenetic tree of Bulinus species with bars, on the right, denoting different species delimitation results, based on the dataset of concatenated cox1 and ITS2 sequences. Within the phylogeny, nodes supported and shared between BI and ML methods are marked with stars where support equates to 90–100% (ML) and 0.95–1 (BI). Names in bold are for specimens collected in the present study and the rest have been retrieved from GenBank: B. truncatus (a); B. tropicus (b); Bulinus sp. 1 (c); Bulinus sp. 2 (d). Locality details are provided in Table 1. Abbreviations: CLI, Clade I; CLII, Clade II; SCI, Subclade I; SCII, Subclade II. The three-letter abbreviation for countries is also given. Notes: the blue colour represents different species, while green stands for the same species according to species delimitation methods. The three-letter abbreviations represent countries: NIG, Nigeria; SAF, South Africa; UGA, Uganda; MLW, Malawi; TZA, Tanzania; CAM, Cameroon; SEN, Senegal; ZNZ, Zanzibar; KEN, Kenya; ANG, Angola. The information for sequences retrieved from the GenBank is presented in Additional file 1: Table S1