Fig. 4.

TNFAIP8 overexpression promotes cell proliferation and drug resistance and decreases apoptosis in chemosensitive cell lines K562 and HL60. a K562 and HL60 were transduced with Flag-tagged TNFAIP8 (TNFAIP8) or control lentivirus (Mock). Cells were sorted by puromycin followed by quantitative PCR and western blots with indicated antibodies. b Proliferation of K562 cells (TNFAIP8 or Mock) and HL60 cells (TNFAIP8 or Mock) was assessed by CCK8 assays, and proliferation rates at 0, 12, 24, 48 and 72 h were calculated normalized to the absorbance at 0 h. c K562 cells (TNFAIP8 or Mock) and HL60 cells (TNFAIP8 or Mock) were treated with ADM (2 μg/mL), Ara-C (10 μM) and IDA (0.05 μg/mL) for 48 h to measure apoptosis by flow cytometry. d IC₅₀ values of K562 cells (TNFAIP8 or Mock) and HL60 cells (TNFAIP8 or Mock) were calculated according to cell growth inhibition after 48 h treatment with serial dilutions of ADM, Ara-C and IDA. Data are mean ± SD values of three independent experiments calculated by Mann-Whitney U test or unpaired Student t-test. * P < 0.05; ** P < 0.01; *** P < 0.001