Table 5.

Validation of sRNAs by stemloop qRT-PCR.

siRNA/reference miRNA	sRNA sequencing
	Abundance control 24 hpb	Abundance control 48 hpb	Abundance dsRNA 24 h	Abundance dsRNA 48 h	Abundance dsDNA 24 h	Abundance dsDNA 48 h
siRNA_186906	0	0	1	0	4	6
siRNA_141931	0	0	0	0	4	9
siRNA_568285	0	0	0	0	1	3
gma-miR166a-3p	432,414	421,684	387,193	363,170	466,733	393,277

siRNA/reference miRNA	Stemloop qRT-PCR
	Ct Control 24 hpb	Ct Control 48 hpb	Ct dsRNA 24 h	Ct dsRNA 48 h	Ct dsDNA 24 h	Ct dsDNA 48 h
siRNA_186906
Rep 1*	33.30	33.18	33.75	33.08	33.17	33.62
siRNA_186906
Rep 1*	33.30	33.18	33.75	33.08	33.17	33.62
Rep 2*	33.55	32.95	33.30	33.04	33.69	33.50
siRNA_141931
Rep 1*	21.48	21.48	22.30	21.37	21.65	22.49
Rep 2*	22.35	21.12	21.81	21.62	22.19	22.35
siRNA_568285
Rep 1*	38.65	38.35	38.08	37.26	39.27	38.68
Rep 2*	39.29	39.14	38.26	37.88	38.67	37.95
gma-miR166a-3p
Rep 1*	26.93	26.83	27.36	27.12	26.99	29.71
Rep 2*	27.07	27.24	27.10	27.92	28.78	29.17

^*Rep: biological replicate.