Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 14;11:4075. doi: 10.1038/s41467-020-17942-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — Bar graph showing the number of LT-HSCs (a), LSK (b) and LK (c) cells in bone marrow of young and old mice as frequency of live single cells (N = 6/condition, six independent experiments). Values are mean ± s.d. ***P < 0.001 (two-tailed unpaired Student’s t test). Exact P values: LT-HSCs: 0.000007; LSK: 0.000013; LK: 0.000039. d Representative flow cytometry plots of myelo-erythroid progenitors from young (top panels) and old mice (bottom panels). e Quantification of myelo-erythroid progenitors from young and old mice (N = 6/condition, six independent experiments). Values are mean ± s.d. *P < 0.05; **P < 0.01; ***P < 0.001 (two-tailed unpaired Student’s t test). Exact P values: MkP: 0.00000006; preGM: 0.0407; GMP: 0.00000008; preMegE: 0.0001; preCFU-E: 0.0002; CFU-E: 0.0010. f In vitro erythroid colony-forming potential of MPP2 (young N = 13, old N = 16), preCFU-E (young N = 17, old N = 18) and CD71⁺ CFU-E (young N = 13, old N = 13) cells from BM of young or old mice (six independent experiments) normalised to colony output from young mice (=100%). Values are mean ± s.e.m. **P < 0.01; ***P < 0.001 (two-tailed unpaired Student’s t test). Exact P values: MPP2: 0.0032; preCFU-E: 0.000005; CFU-E CD71+: 0.000003. g GSEA analysis comparing expression of preCFU-E-specific genes in young and old preCFU-Es. The normalised enrichment score (NES) and P value are shown. h GSEA analysis comparing expression of preGM-specific genes in young and old preCFU-Es. The normalised enrichment score (NES) and P value are shown. i In vitro myeloid colony-forming potential of MPP2 cells (young: N = 7, old: N = 10; two independent experiments) and preCFU-E cells (N = 6/condition, six independent experiments) from BM of young and old mice normalised to colony output from young mice (=100%). Values are mean ± s.e.m. ***P < 0.001 (two-tailed unpaired Student’s t test). Exact P values: MPP2: 0.000022; preCFU-E: 0.0009. Source data are provided as a source data file.